Mutual genetic antagonism involving GLI3 and dHAND prepatterns the vertebrate limb bud mesenchyme prior to SHH signaling
P te Welscher, M Fernandez-Teran… - Genes & …, 2002 - genesdev.cshlp.org
P te Welscher, M Fernandez-Teran, MA Ros, R Zeller
Genes & development, 2002•genesdev.cshlp.orgThe bHLH transcription factor dHAND is required for establishment of SHH signaling by the
limb bud organizer in posterior mesenchyme, a step crucial to development of vertebrate
paired appendages. We show that the transcriptional repressor GLI3 restricts dHAND
expression to posterior mesenchyme prior to activation of SHH signaling in mouse limb
buds. dHAND, in turn, excludes anterior genes such as Gli3 and Alx4 from posterior
mesenchyme. Furthermore, genetic interaction of GLI3 and dHAND directs establishment of …
limb bud organizer in posterior mesenchyme, a step crucial to development of vertebrate
paired appendages. We show that the transcriptional repressor GLI3 restricts dHAND
expression to posterior mesenchyme prior to activation of SHH signaling in mouse limb
buds. dHAND, in turn, excludes anterior genes such as Gli3 and Alx4 from posterior
mesenchyme. Furthermore, genetic interaction of GLI3 and dHAND directs establishment of …
The bHLH transcription factor dHAND is required for establishment of SHH signaling by the limb bud organizer in posterior mesenchyme, a step crucial to development of vertebrate paired appendages. We show that the transcriptional repressor GLI3 restricts dHANDexpression to posterior mesenchyme prior to activation of SHH signaling in mouse limb buds. dHAND, in turn, excludes anterior genes such asGli3 and Alx4 from posterior mesenchyme. Furthermore, genetic interaction of GLI3 and dHAND directs establishment of the SHH/FGF signaling feedback loop by restricting the BMP antagonist GREMLIN posteriorly. These interactions polarize the nascent limb bud mesenchyme prior to SHH signaling.
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