[HTML][HTML] Dual phosphorylation of suppressor of fused (Sufu) by PKA and GSK3β regulates its stability and localization in the primary cilium
Y Chen, S Yue, L Xie, X Pu, T Jin, SY Cheng - Journal of Biological …, 2011 - ASBMB
Suppressor of fused (Sufu) is an essential negative regulator of the sonic hedgehog (Shh)
pathway, but little is known about how Sufu itself is normally regulated. Here, we report that
Sufu is phosphorylated at Ser-342 and Ser-346 by GSK3β and cAMP-dependent protein
kinase A (PKA), respectively, and phosphorylation at this dual site stabilizes Sufu against
Shh signaling-induced degradation. We further show that localization of Sufu in the primary
cilium is induced by Shh signaling and is required for the turnover of both phosphorylated …
pathway, but little is known about how Sufu itself is normally regulated. Here, we report that
Sufu is phosphorylated at Ser-342 and Ser-346 by GSK3β and cAMP-dependent protein
kinase A (PKA), respectively, and phosphorylation at this dual site stabilizes Sufu against
Shh signaling-induced degradation. We further show that localization of Sufu in the primary
cilium is induced by Shh signaling and is required for the turnover of both phosphorylated …