Preparation of primary cultures of embryo fibroblasts from genetically engineered mouse
strains can provide a valuable resource for analyzing the consequences of genetic
alterations at the cellular level. Mouse embryo fibroblasts (MEFs) have been particularly
useful in cancer research, as they have facilitated the identification of the genetic changes
that allow cells to overcome senescence and proliferate indefinitely in culture. The
immortalized MEFs can then acquire additional mutations that lead to anchorage …