RIP3 AND pMLKL promote necroptosis-induced inflammation and alter membrane permeability in intestinal epithelial cells
A Negroni, E Colantoni, M Pierdomenico… - Digestive and Liver …, 2017 - Elsevier
Background Necroptosis is an inflammatory form of programmed cell death requiring
receptor-interacting protein kinase 3 (RIP3) and mixed lineage kinase domain-like protein
(MLKL). Aims The aim of this study is to examine in depth in vitro and ex vivo the contribution
of necroptosis to intestinal inflammation. Methods In vitro: we used an intestinal cell line,
HCT116RIP3, produced in our laboratory and overexpressing RIP3. Ex vivo: intestinal
mucosal biopsies were taken from patients with inflammatory bowel disease (IBD)(20 with …
receptor-interacting protein kinase 3 (RIP3) and mixed lineage kinase domain-like protein
(MLKL). Aims The aim of this study is to examine in depth in vitro and ex vivo the contribution
of necroptosis to intestinal inflammation. Methods In vitro: we used an intestinal cell line,
HCT116RIP3, produced in our laboratory and overexpressing RIP3. Ex vivo: intestinal
mucosal biopsies were taken from patients with inflammatory bowel disease (IBD)(20 with …