Assays to monitor the metabolic state or nutrient uptake capacity of immune cells at a single
cell level are increasingly in demand. One assay, used by many immunologists, employs 2-
(N-(7-Nitrobenz-2-oxa-1, 3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG), a fluorescent
analogue of 2-deoxyglucose (2DG), as a substrate for glucose transporters. This molecule
has been validated as a substrate for the glucose transporter Glut2 (Slc2a2) in mammalian
cells but 2-NDBG selectivity for the glucose transporters expressed by T cells, Glut1 (Slc2a1) …