Reactive oxygen species alter gene expression in podocytes: induction of granulocyte macrophage-colony-stimulating factor
S Greiber, B Mu, P Daemisch… - Journal of the American …, 2002 - journals.lww.com
It has been suggested that reactive oxygen radicals (ROS) play a crucial role in the
pathogenesis of proteinuria and podocyte injury. It was investigated whether changes in
gene expression might account for ROS-induced podocyte dysfunction. Differentiated
podocytes were incubated with control media or with exogenous ROS from the
xanthine/xanthine-oxidase reaction for 4 h. A PCR-based suppressive subtractive
hybridization assay was applied to isolate and clone mRNAs that were differentially …
pathogenesis of proteinuria and podocyte injury. It was investigated whether changes in
gene expression might account for ROS-induced podocyte dysfunction. Differentiated
podocytes were incubated with control media or with exogenous ROS from the
xanthine/xanthine-oxidase reaction for 4 h. A PCR-based suppressive subtractive
hybridization assay was applied to isolate and clone mRNAs that were differentially …
