Depletion of reduced glutathione precedes inactivation of mitochondrial enzymes following limbic status epilepticus in the rat hippocampus
The time course and critical determinants of mitochondrial dysfunction and oxidative stress
following limbic status epilepticus (SE) were investigated in hippocampal sub-regions of an
electrical stimulation model in rats, at time points 4–44h after status. Mitochondrial and
cytosolic enzyme activities were measured spectrophotometrically, and reduced glutathione
(GSH) concentrations by HPLC, and compared to results from sham controls. The earliest
change in any sub-region was a fall in GSH, appearing as early as 4h in CA3 (− 13%, p< …
following limbic status epilepticus (SE) were investigated in hippocampal sub-regions of an
electrical stimulation model in rats, at time points 4–44h after status. Mitochondrial and
cytosolic enzyme activities were measured spectrophotometrically, and reduced glutathione
(GSH) concentrations by HPLC, and compared to results from sham controls. The earliest
change in any sub-region was a fall in GSH, appearing as early as 4h in CA3 (− 13%, p< …