[HTML][HTML] Characterization of cultured cholangiocytes isolated from livers of patients with primary sclerosing cholangitis
JH Tabibian, CE Trussoni, SP O'hara, PL Splinter… - Laboratory …, 2014 - Elsevier
Primary sclerosing cholangitis (PSC) is a chronic, idiopathic cholangiopathy. The role of
cholangiocytes (biliary epithelial cells) in PSC pathogenesis is unknown and remains an
active area of research. Here, through cellular, molecular and next-generation sequencing
(NGS) methods, we characterize and identify phenotypic and signaling features of isolated
PSC patient-derived cholangiocytes. We isolated cholangiocytes from stage 4 PSC patient
liver explants by dissection, differential filtration and immune-magnetic bead separation. We …
cholangiocytes (biliary epithelial cells) in PSC pathogenesis is unknown and remains an
active area of research. Here, through cellular, molecular and next-generation sequencing
(NGS) methods, we characterize and identify phenotypic and signaling features of isolated
PSC patient-derived cholangiocytes. We isolated cholangiocytes from stage 4 PSC patient
liver explants by dissection, differential filtration and immune-magnetic bead separation. We …
