[HTML][HTML] Studies on the substrate and stereo/regioselectivity of adipose triglyceride lipase, hormone-sensitive lipase, and diacylglycerol-O-acyltransferases
Adipose triglyceride lipase (ATGL) is rate-limiting for the initial step of triacylglycerol (TAG)
hydrolysis, generating diacylglycerol (DAG) and fatty acids. DAG exists in three
stereochemical isoforms. Here we show that ATGL exhibits a strong preference for the
hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone. The
selectivity of ATGL broadens to the sn-1 position upon stimulation of the enzyme by its co-
activator CGI-58. sn-1, 3 DAG is the preferred substrate for the consecutive hydrolysis by …
hydrolysis, generating diacylglycerol (DAG) and fatty acids. DAG exists in three
stereochemical isoforms. Here we show that ATGL exhibits a strong preference for the
hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone. The
selectivity of ATGL broadens to the sn-1 position upon stimulation of the enzyme by its co-
activator CGI-58. sn-1, 3 DAG is the preferred substrate for the consecutive hydrolysis by …
