Heterotopic bone formation about the hip undergoes endochondral ossification: a rabbit model
O Tannous, AC Stall, C Griffith, CT Donaldson… - … and Related Research®, 2013 - Springer
O Tannous, AC Stall, C Griffith, CT Donaldson, RJ Castellani, VD Pellegrini
Clinical Orthopaedics and Related Research®, 2013•SpringerBackground Heterotopic ossification (HO) occurs most commonly after trauma and surgery
about the hip and may compromise subsequent function. Currently available animal models
describing the cellular progression of HO are based on exogenous osteogenic induction
agents and may not reflect the processes following trauma. Questions/purposes We
therefore sought to characterize the histologic progression of heterotopic bone formation in
an animal model that recapitulates the human condition without the addition of exogenous …
about the hip and may compromise subsequent function. Currently available animal models
describing the cellular progression of HO are based on exogenous osteogenic induction
agents and may not reflect the processes following trauma. Questions/purposes We
therefore sought to characterize the histologic progression of heterotopic bone formation in
an animal model that recapitulates the human condition without the addition of exogenous …
Background
Heterotopic ossification (HO) occurs most commonly after trauma and surgery about the hip and may compromise subsequent function. Currently available animal models describing the cellular progression of HO are based on exogenous osteogenic induction agents and may not reflect the processes following trauma.
Questions/purposes
We therefore sought to characterize the histologic progression of heterotopic bone formation in an animal model that recapitulates the human condition without the addition of exogenous osteogenic material.
Methods
We used a rabbit model that included intramedullary instrumentation of the upper femur and ischemic crush injury of the gluteal muscle. Bilateral surgical induction procedures were performed on 30 animals with the intention of inciting the process of HO; no supplemental osteogenic stimulants were used. Three animals were sacrificed at each of 10 predetermined times between 1 day and 26 weeks postoperatively and the progression of tissue maturation was graded histologically using a five-item scale.
Results
Heterotopic bone reliably formed de novo and consistently followed a pathway of endochondral ossification. Chondroid elements were found in juxtaposition with immature woven bone in all sections that contained mature osseous elements.
Conclusions
These results establish that HO occurs in an animal model mimicking the human condition following surgical trauma about the hip; it is predictable in its histologic progression and follows a pathway of endochondral bone formation.
Clinical Relevance
By showing a consistent pathway of endochondral ossification leading to ectopic bone formation, this study provides a basis for understanding the mechanisms by which HO might be mitigated by interventions.
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